DIFFRACTION PHASE AND FLUORESCENCE MICROSCOPY, OPT. EXP. 14, 8263, (2006)

Y. K. PARK, G. POPESCU, K. BADIZADEGAN, R. R. DASARI AND M. S. FELD
2006

We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells.