whitelight Diffraction Phase Microscopy (wDPM)

Whitelight Diffraction Phase Microscopy upgrades research-grade optical microscopes to provide FAST 4D label-free quantitative imaging of optically thin specimens harnessing the full camera speed capabilities (e.g. hundreds of frames per second for high resolution sCMOS sensors). 

wDPM specialized applications include:

  • mapping refractive index changes in glass substrates under laser bombardment
  • high throughput measurements (size and count) of cells, protein particles and aggregates
wDPM cell culture imaging

wDPM cell culture imaging


wDPM advantages

wDPM z-scan of femtosecond laser induced changes in glass substrates

Phi Optics wDPM seamlessly upgrades new and existing commercial (Zeiss, Nikon, Leica, Olympus) microscopes with any magnification available (immersion or dry, 5X to 100X). Samples can be loaded in standard holders (glass slides, single and multi-well plates) and fields of view are limited only by the microscope stage movement.  
High end sCMOS and EMCCD cameras can be integrated for high sensitivity fluorescence co-localization. Any fluorescence channels present on the microscope can be used for imaging.

wDPM versions:

Phi Optics implements wDPM as an add-on module that connects to the camera port of a phase contrast microscope. Phi Optics CellVista acquisition platform controls the motorization of the microscope (stage, Z-drive, fluorescence turret, shutters). CellVista can acquire programmed 4D (3D time-series) multi-channel images of the samples including wDPM and fluorescence. All output is captured with the same camera for seamless channel overlay. 

Phi Optics whitelight Diffraction Phase Microscopy (wDPM) imaging system comes in three different versions: wDPM Basic, wDPM Pro and wDPM Ultimate.

wDPM Basic works with manual microscope stands, therefore is suitable for single-location, single-focus time-lapse imaging.

wDPM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning. SLIM Pro is also suitable for multi-channel experiments and fast dynamics measurement. 

At full speed, wDPM Pro generates more than 1TB of data per hour. For efficient management of this high volume of data acquired over a long period of time (weeks/months) we recommend wDPM Ultimate. wDPM Ultimate combines SLIM Pro with a vast storage server of 100TB.

wDPM with FLIR camera on Zeiss microscope

CellVista wDPM PRO module attached to a Zeiss Axio Observer microscope (Microscope sold separately.)

Phi Optics wDPM-based products:

Real-Time Imaging Yes Yes Yes
Programmed 2D Scanning No Yes Yes
Programmed 3D Scanning No Yes Yes
Fluorescence Overlay No Yes Yes
Large HDD array No No Yes
wDPM Acquisition Speed: 30 fps @1024×1024 px 200 fps @1024×1024 px
800 fps @256×256 px
200 fps @1024×1024 px
800 fps @256×256 px

For a high image resolution solution for cell assays please see our Spatial Light Interference Microscopy (SLIM) systems

For a 3D tomography solution for thick specimens please see our Gradient Light Interference Microscopy (GLIM) systems

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