Elevate your next proposal using “Quantitative, high resolution 3D tomography of optically thick specimens with GLIM”: new webinar on October 1st!

We are happy to announce our next webinar with the topic “Quantitative, high resolution 3D tomography of optically thick specimens (tissues, organoids, embryos, animal models) with Phi Optics GLIM” on October 1, 2020. The webinar is hosted by AXT Pty Ltd, exclusive distributor of Phi Optics instruments in Australia and New Zealand.

Dr. Gabriel Popescu, Professor at UIUC Beckman Institute, will discuss the challenges in imaging in-vitro 3D structures with current optical methods due to loss of contrast caused by multiple scattering. Gradient Light Interference Microscopy (GLIM) rejects much of the multiple scattering contributions and exhibits strong optical sectioning needed for 3D label-free and quantitative imaging of these specimens. Dr. Popescu will illustrate the use of GLIM technology in visualization of 3D time-lapse evolution of optically thick specimens such as organoids, embryos, engineered tissues and small model animals (zebra fish, C. elegans), including results published in Nature Communications in 2017 and 2019.

Bovine embryo reconstruction with GLIM
Bovine embryo reconstruction with GLIM (with permission from Nguyen, Kandel et al, Nat Commun 8, 210 (2017))

Presenter – Dr. Gabriel Popescu
Professor, Electrical and Computer Engineering, University of Illinois at Urbana Champaign

Prof. Gabriel Popescu, UIUC Beckman, QLI Lab

Following his BS and MS in Physics from University of Bucharest, he received his Ph.D. in Optics in 2002 from the School of Optics/ CREOL (now the College of Optics and Photonics), University of Central Florida. He continued his training with the late Michael Feld at M.I.T., working as a postdoctoral associate. He joined Illinois in August 2007 where he directs the Quantitative Light Imaging Laboratory (QLI Lab) at the Beckman Institute for Advanced Science and Technology. Dr. Popescu served as Associate Editor of Optics Express and Biomedical Optics Express, Editorial Board Member for Journal of Biomedical Optics and Scientific Reports. He authored three books, edited another book, authored 185 journal publications, 230 conference presentations, 32 patents, gave 220 lecture/plenary/invited talks. He founded Phi Optics, Inc., a start-up company that commercializes quantitative phase imaging technology. He is a Fellow of OSA, SPIE, AIMBE, and senior member of IEEE.

Phi Optics implements GLIM technology into a compact add-on module that connects to the camera port of the microscope frame. GLIM modules can image with any magnification from 1X to 100X, dry or immersion objectives. Phi Optics CellVista software suite controls the microscope motorization to provide 4D (3D time lapse) multi-channel imaging including fluorescence. The same camera is used for GLIM and fluorescence channels for seamless image registration. GLIM’s exceptional sensitivity and SNR can be combined with machine-learning algorithms for digital staining of live specimens: Phase Imaging with Computational Specificity (PICS).

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