GLIM Gradient Light Interference Microscopy

Widefield 3D tomography

Multiple scattering limits the contrast in optical imaging of thick specimens. Gradient Light Interference Microscopy (GLIM) is a new quantitative phase imaging technique that combines multiple intensity images that correspond to controlled phase shifts between two interfering waves, thereby suppressing the incoherent background due to multiple scattering. GLIM exploits a special case of low-coherence interferometry to extract phase information from 2D and 3D samples, which in turn can be used to measure cell mass, volume, surface area, and their evolutions in time. As an add-on module, GLIM holistically improves the contrast in a DIC microscope enabling quantitative measurements of live specimens.


Phi Optics Technology Provides

• Non-invasive: no sample preparation. (Resolves samples with thickness from 50 µm – 350 µm+)
• Quantitative measurements: thickness and dry mass
• Label free continuous imaging from milliseconds to days
• Integrates with existing research grade microscopes
• Programmed 4D (tiling, z-scan, time series) scanning and acquisition at up to 12fps with full camera resolution
• Multichannel imaging (including fluorescence channels) with seamless overlay
• ImageJ-based toolkit for measurement and 3D image rendering

Applications Include

Brain sections

3D Organoids


Animal Models (worms, zebrafish, fruit flies)


GLIM module with camera easily attaches to an open C-Mount of existing inverted DIC microscope.

How It Works