GLIM

GLIM-Zeiss-HamGradient Light Interference Microscopy (GLIM), attached to a Zeiss Axio Observer microscope with DIC illumination. (Microscope sold separately.)

The Gradient Light Interference Microscopy (“GLIM”) imaging system comes in two different versions: GLIM Basic and GLIM Pro. GLIM Basic works with manual microscope stands and is suitable for single-location, single-focus time-lapse imaging. GLIM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement. At full speed, GLIM Pro generates more than 1TB of data per hour. Phi Optics offers storage server solutions for efficient management of this high volume of data. Please contact us with inquiries.

GLIM FEATURES

  • Resolves samples with thickness from 50 µm – 350 µm+
  • Quantitative measurements: thickness and dry mass
  • Label free continuous imaging from milliseconds to days
  • Non-invasive: no sample preparation.
  • Integrates with existing research grade microscopes
  • Programmed 4D (tiling, z-scan, time series) scanning and acquisition at up to 12 fps with full camera resolution
  • Multichannel imaging (including fluorescence channels) with seamless overlay
  •  ImageJ-based toolkit for measurement and 3D image rendering

GLIM2 Leica FLIR 11.18   

GLIM system is available in two versions, the Basic (left) and Pro. Both include a module, computer, software and camera. GLIM attaches to an existing inverted DIC microscope, sold separately.

FEATURE GLIM BASIC GLIM PRO
Real-Time Imaging Yes Yes
Programmed 2D Scanning No Yes
Programmed 3D Scanning No Yes
Programmed Fluorescence Overlay No Yes
SLIM Acquisition Speed: Up to 4 fps (1928 x 1448) Up to 12 fps (2048 x 2048)

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