Products

SLIM – SPATIAL LIGHT INTERFERENCE MICROSCOPY

SLIM-Nikon-Andor

Spatial Light Interference Microscopy (SLIM) module connected to a Nikon Ti2 inverted optical microscope. (Microscope sold separately.)

Spatial Light Interference Microscopy (SLIM) provides 4D quantitative, real-time and non-destructive imaging of live cell cultures, microorganisms (mycoplasma), and reconstituted protein solutions. The SLIM module works in conjunction with an existing optical microscope, and is available in three different versions: SLIM Basic, SLIM Pro and SLIM Ultimate.

SLIM Basic works with manual microscope stands and is suitable for single-location, single-focus time-lapse imaging.

SLIM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement. At full speed, SLIM Pro generates more than 1TB of data per hour.

For efficient management of this high volume of data, SLIM Ultimate combines SLIM Pro with a vast storage server of 100TB. This is a customized solution. Please inquire for more details and a quote. 

GLIM – GRADIENT LIGHT INTERFERENCE MICROSCOPY

 

GLIM-Leica-HamGradient Light Interference Microscopy (GLIM) module connected to a Leica DMi8 with DIC illumination. (Microscope sold separately.)

GLIM provides quantitative, high resolution 4D tomography of optically thick specimens such as cell aggregates, bacterial colonies, 3D organoids, brain slices, embryos, and model multicellular organisms.

The GLIM imaging system comes in two different versions: GLIM Basic and  GLIM Pro.

GLIM Basic comes with compact geometry. It connects via C-mount to microscope stands via an available camera port. GLIM Basic can function with either motorized or manual microscopes. On a manual microscope, GLIM Basic is suitable for single-location, single-focus time-lapse imaging, which can be a less expensive entry point for QPI imaging. 

For more capabilities, GLIM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement. At full speed, GLIM Pro generates more than 1TB of data per hour.

Phi Optics offers customized storage server solutions for efficient management of high volume data requirements for long-term live QPI  experiments. Please inquire directly for more information and a quote. 

wDPM – WHITELIGHT DIFFRACTION PHASE MICROSCOPY

wDPM Zeiss FLIR

Whitelight Diffraction Phase Microscopy (wDPM) module connected to a Zeiss Observer with BF illumination. (Microscope sold separately.)

Whitelight Diffraction Phase Microscopy (wDPM) provides 3D quantitative, non-destructive imaging of live cell cultures, microorganisms (mycoplasma), and reconstituted protein solutions at the full speed of the camera. Due to its high framerate, wDPM can monitor material changes generated by femtosecond laser pulses focused in the volume of transparent materials: quantitative phase and amplitude associated with local change in refractive index or absorption, or cavitation phenomena in a liquid medium. 

The wDPM module works in conjunction with an existing optical microscope, and is available in three different versions: wDPM Basic, wDPM Pro and wDPM Ultimate.

wDPM Basic works with manual microscope stands and is suitable for single-location, single-focus time-lapse imaging.

wDPM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement.

wDPM Ultimate combines wDPM Pro with a vast storage server of 100TB. This is a customized solution. Please inquire for more details and a quote.

CELLVISTA image acquisition software

Phi Optics QPI instruments are operated by our proprietary CellVista imaging platform. CellVista is Windows-based and easy to use, but it can handle complex experiments and help optimize your time in the lab. CellVista controls the motorization of the microscope (stage, Z-drive, shutters, filter turret) to acquire 3D and time-lapse multichannel images of the samples: all the regular channels of the microscope (brightfield, phase contrast, DIC, fluorescence) and QPI (SLIM, GLIM or wDPM).  All channels are captured with same camera for seamless overlay.

The output is TIFF files for each 4D (x-y-z + time) location. in QPI images pixel intensity is proportional to the optical path length (OPL) change (in radians). In regular channel images (e.g. fluorescence) pixel intensity is a measure of sample signal. TIFF files are floating point (32-bit for QPI images, 16-bit for regular channels) and are suitable for machine learning or big data projects.

A CellVista SDK can be used to integrate the QPI modules in other image acquisition platforms. The SDK has already been tested with MD MetaMorph®, Open Imaging Micro-Manager, and NI LabView™.

 

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