Phi Optics SLIM, GLIM and wDPM instruments for label-free and modular quantitative microscopy.
SLIM – SPATIAL LIGHT INTERFERENCE MICROSCOPY
Spatial Light Interference Microscopy (SLIM) module connected to a Nikon Ti2 inverted optical microscope. (Microscope sold separately)
Spatial Light Interference Microscopy (SLIM) is implemented into a modular microscope add-on that provides 4D quantitative, real-time and non-destructive imaging of live cell cultures, microorganisms (mycoplasma), and reconstituted protein solutions.
GLIM – GRADIENT LIGHT INTERFERENCE MICROSCOPY
Gradient Light Interference Microscopy (GLIM) module connected to a Leica DMi8 with DIC illumination. (Microscope sold separately.)
Gradient Light Interference Microscopy (GLIM) is implemented into a modular microscope add-on that provides quantitative, high resolution 4D tomography of optically thick specimens such as cell aggregates, bacterial colonies, 3D organoids, brain slices, embryos, and model multicellular organisms.
wDPM – WHITELIGHT DIFFRACTION PHASE MICROSCOPY
Whitelight Diffraction Phase Microscopy (wDPM) module connected to a Zeiss Observer with BF illumination. (Microscope sold separately.)
Whitelight Diffraction Phase Microscopy (wDPM) is implemented into a modular microscope add-on that provides 3D quantitative, non-destructive imaging of live cell cultures, microorganisms (mycoplasma), and reconstituted protein solutions at the full speed of the camera. Due to its high framerate, wDPM can monitor material changes generated by femtosecond laser pulses focused in the volume of transparent materials: quantitative phase and amplitude associated with local change in refractive index or absorption, or cavitation phenomena in a liquid medium.
CELLVISTA image acquisition software
Phi Optics QPI instruments are operated by our proprietary CellVista imaging platform. CellVista is Windows-based and easy to use, but it can handle complex experiments and help optimize your time in the lab. CellVista controls the motorization of the microscope (stage, Z-drive, shutters, filter turret) to acquire 3D and time-lapse multichannel images of the samples: all the regular channels of the microscope (brightfield, phase contrast, DIC, fluorescence) and QPI (SLIM, GLIM or wDPM). All channels are captured with same camera for seamless overlay.
The output is TIFF files for each 4D (x-y-z + time) location. in QPI images pixel intensity is proportional to the optical path length (OPL) change (in radians). In regular channel images (e.g. fluorescence) pixel intensity is a measure of sample signal. TIFF files are floating point (32-bit for QPI images, 16-bit for regular channels) and are suitable for machine learning or big data projects.
A CellVista SDK can be used to integrate the QPI modules in other image acquisition platforms. The SDK has already been tested with MD MetaMorph®, Open Imaging Micro-Manager, and NI LabView™.
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