Phase Rings with SLIM

The three variable phase rings shifts the phase of the reference beam by a fixed amount (0, 0.5pi, pi, 1.5pi) and the camera captures the resulting frame.

The SLIM module relays the image plane with minimal aberrations (diffraction limited) at a 1:1 ratio to a camera sitting at its exit port. The active element at the heart of the SLIM module is a liquid crystal spatial light modulator (SLM). The SLM is conjugated with the back focal plane of the microscope objective, and it modulates the reference beam like a phase plate with variable thickness. To create a quantitative phase image the SLM works as a tunable phase ring and shifts the phase of the reference beam by a fixed amount (0, 0.5pi, pi, 1.5pi) and the camera captures the resulting frame (Figure b). The CellVista software module combines the four frames by solving the field interference equations in each point of the frame – the result (Figure c) is a quantitative-phase (SLIM) image that is uniquely determined.

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