GLIM Rendering

The GLIM module is designed as an add-on module connected to the output port of an inverted DIC microscope. This module shifts the phase of one polarization component using an SLM while keeping the other unmodified. Interference patterns generated by these two components are recorded and transferred to a computer for phase gradient extraction. Four frames are acquired by the GLIM module, one for each phase shift applied by the SLM. Using these images, we obtain the phase gradient map and integrate it along the direction of the shift to recover the quantitative phase map.

GLIM complements SLIM to provide 3D tomographic imaging of live, optically thick biospecimens at high contrast (a). The GLIM module acquires four frames, one for each phase shift applied by the SLM (b). From the four frames, GLIM constructs quantitative phase-gradient maps (c). This GLIM quantitative phase image was obtained by integration along the direction of the shift phase (d). Cross-sections of the reconstructed phase and the computed ground truth (black dashed curve) are also shown (e). (Adapted with permission from