SLIM

Phi Optics SLIM comes in three different versions: SLIM Basic, SLIM Pro and SLIM Ultimate. SLIM Basic works with manual microscope stands and is suitable for single-location, single-focus time-lapse imaging. SLIM Pro interfaces with motorized microscope stands to yield fully automated high-throughput, multichannel acquisition. It is suitable for more complicated projects that require 2D and 3D scanning, multi-channel experiments and fast dynamics measurement. At full speed, SLIM Pro generates more than 1TB of data per hour. For efficient management of this high volume of data, SLIM Ultimate combines SLIM Pro with a vast storage server of 100TB.

OVERVIEW

Phi Optics technology provides:

Real-time quantitative phase imaging and display

Non-invasive, label-free cell and tissue imaging on broad time scales (seconds to weeks)

Programmed 2D and 3D scanning for large area 2D SLIM maps and 3D SLIM images

Seamless overlay with other microscopy channels

Quick and easy segmentation of cells

Applications Include:

Cell growth

Cell dynamics

3D tomography

Neuroscience

Blood testing

Tissue imaging

HOW IT WORKS

Schematic setup for SLIM

Spatial Light Interference Microscopy (SLIM)  is a non-invasive phase imaging technology that quantifies the optical path length differences in a biospecimen and converts them into thickness, dry mass area density and refractive index maps. Figure a) illustrates the principle of the technology. A live cell in culture medium is imaged with the phase contrast modality of the microscope : the light passing through the object (scattered beam) and the light passing through the medium (reference beam) combine through interference in the image plane. The optical path length differences between the beams (i.e. the phase shift) in each point of the image plane are measured. The measured phase shift is proportional to the optical density at each point in space. In other words, optically denser areas of the cell (e.g. nucleus) introduce a larger phase shift.

The phase rings and their corresponding images recorded by the CCD.

The SLIM module relays the image plane with minimal aberrations (diffraction limited) at a 1:1 ratio to a camera sitting at its exit port. The active element at the heart of the SLIM module is a liquid crystal spatial light modulator (SLM). The SLM is conjugated with the back focal plane of the microscope objective, and it modulates the reference beam like a phase plate with variable thickness. To create a quantitative phase image the SLM works as a tunable phase ring and shifts the phase of the reference beam by a fixed amount (0, 0.5pi, pi, 1.5pi) and the camera captures the resulting frame (Figure b). The CellVista software module combines the four frames by solving the field interference equations in each point of the frame – the result (Figure c) is a quantitative-phase (SLIM) image that is uniquely determined.

Reconst

SLIM is a wide field quantitative imaging method thus it can measure simultaneously large populations of cells at full camera resolution (e.g. 2 mm FOV for 10X objective at 4.2 MP camera resolution). Wide field optical sectioning (e.g. 850 nm Z-resolution for 100X/1.4NA objective) enables 3D tomography. All microscope output is acquired with the same camera which enables seamless overlay of SLIM images with fluorescence channels.

PRODUCTS

SLIM Basic

SLIM PRO

Slim Ultimate

Real-Time Imaging Yes Yes Yes
Programmed 2D Scanning No Yes Yes
Programmed 3D Scanning No Yes Yes
Programmed Fluorescence Overlay No Yes Yes
SLIM Acquisition Speed: Up to 4 fps (1928 x 1448) Up to 12 fps (2048 x 2048) Up to 12 fps (2048 x 2048)
Camera: Point Grey USB CCD, 1928 x 1448 Resolution, 26 fps,14 bit Hamamatsu ORCA-Flash 4.0V3, 2048 x 2048 Resolution, 100 fps, 16 bit Hamamatsu ORCA-Flash 4.0V3, 2048 x 2048 Resolution, 100 fps, 16 bit
Computer & Storage: Intel Core i7, 16GB RAM, 1TB HDD, NVIDIA GeForce GTX 780, Windows 7 Intel Core i7, 32GB RAM, 1TB SSD + 2TB HDD, NVIDIA GeForce GTX 780, Windows 7 Intel Core i7, 32GB RAM, 2TB SSD + 100TB HDD, NVIDIA GeForce GTX 780, Windows 7
Software: Acquisition: CellVista Basic Postprocessing: ImageJ Phi Optics Toolbox Acquisition: CellVista Pro Postprocessing: ImageJ Phi Optics Toolbox Acquisition: CellVista Pro Postprocessing: ImageJ Phi Optics Toolbox
Hardware Footprint: 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H) 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H) 43 x 36 x 11 cm / 17 x 14 x 4 in (L x W x H)
Measurement Resolution: Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)
Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)
Path Length Sensitivity < 1 nm
Transverse Resolution – diffraction limited (e.g. 350 nm with 63X/1.4NA objective)
Axial Resolution – optical sectioning (e.g. 890 nm for 63X/1.4NA objective)