SLIM combined with DPS for Label-Free 3D Mass Transport

The SLIM add?on (B) enhances a phase contrast microscope (A) by measuring the phase?shift associated with the object transmission function. As in phase contrast, the total field on the detector (U) is a superposition of the transmitted (Udc) and sample perturbed fields (Uac) (C). In our design, a phase ring is matched to an external pupil plane (D), and Uac is modulated in ?/2 increments by a spatial light modulator (SLM). From four such images, we recover the phase shift due to the object (?).

The SLIM system is added to a phase contrast microscope to enhance imaging by measuring and quantifying the the phase?shift associated with the object transmission function.

03-16-2017 | APPLICATION NEWS: Phi Optics SLIM combined with dispersion-relation phase spectroscopy (DPS) is used to study the 3D transport of mass inside neuron bodies and neurites as a function of time and without fluorescence tags.

 

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