Cell growth


Cycle dependent growth of U2OS cell measured using SLIM (M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. Prasanth and G.Popescu, Optical Measurement of cycle-dependent growth , Proc. Natl. Acad. Sci., 108 (32), 13124 (2011))

Spatial Light Interference Microscopy (SLIM)  measures the dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells. Combining SLIM with fluorescence imaging provides a unique method for studying cell cycle-dependent growth: the fluorescent reporter indicates the cell growth stage and SLIM provides non-invasive measurements of cell dry mass with single cell resolution (femtogram sensitivity).


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