Applications

QPI offers label-free, quantitative, 3D, real-time imaging of dynamic processes with nanoscale sensitivity. Whether you are a life sciences researcher or a materials engineer looking for the next technology that will make your work faster and more accurate, QPI has the answer to it – please contact us  with your questions.

Cell Growth

Spatial Light Interference Microscopy (SLIM)  measures the dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells. Combining SLIM with fluorescence imaging provides a unique method for studying cell cycle-dependent growth: the fluorescent reporter indicates the cell growth stage and SLIM provides non-invasive measurements of cell dry mass with single cell resolution (femtogram sensitivity).

Cell Dynamics

Spatial Light Interference Microscopy (SLIM) for studying intra- and inter-cellular dynamics by tracking the movement of small organelles or analyzing a region of interest as a whole. Sub-nanometer sensitivity and diffraction limited resolution of SLIM provide ideal means to study the cell dynamics. The quantitative data yield actual mass transport information, in terms of diffusion coefficients and velocity distributions.

3D Tomography

Spatial Light Interference Microscopy (SLIM) for tomographic imaging and 3D analysis of live cells. Tomography of unlabeled live cells is obtained by scanning the focus through the sample, and sequentially taking a stack of image slices along the cell. The obtained stack holds full-3D information of the object, which can be processed further to obtain information regarding the structure and spatial distribution of the object. 3D rendering of the image stack is done on ImageJ to provide a full and flexible view of a cell. Based on the quantitative phase images, this capability expands the applications of SLIM to science and clinic to another dimension.

Neuroscience

Spatial Light Interference Microscopy (SLIM) is suitable for studying Neuroscience. The non-invasive live cell imaging provides a viable environment for fragile neurons and neuronal stem cells, which are very susceptible to damages from temperature, chemicals, and light. The speed of SLIM acquisition is capable of detecting the transport between neurons, and the wide field of view is capable of imaging the formation of a neuronal network. Therefore, SLIM provides an environment where neurons can be studied at both single cell level and population level.

Blood Testing

Spatial Light Interference Microscopy (SLIM) can be used for blood testing. SLIM has a capability to measure the red blood cell (RBC) thickness, which provides reliable means to infer the condition of the RBCs. The parameters available from SLIM analysis of RBCs include: perimeter, projected area, circular diameter, surface-area, volume, sphericity, eccentricity, minimum height, maximum height, mean height, minimum cylindrical diameter, circularity, integrated density, kurtosis, skewness and variance. When combined with regular bright-field imaging for absorption, SLIM can also infer the information regarding hemoglobin concentration as well.

Tissue Imaging

Spatial Light Interference Microscopy (SLIM) can be used for tissue imaging and diagnosis. SLIM is capable of automatically scanning and imaging microscopy slides with tissue samples. The quantitative data obtained from Phi Optics SLIM provides insight into the condition of the tissue, whether it is in benign or malignant condition, without staining. Therefore, it allows for an automatic diagnosis of cancer in the tissue sample. Moreover, phase imaging reveals certain structures that are not readily detected in simple staining methods that are current standards in clinics that can be used as an easier and faster indicator to diagnose the condition.